The indicators quin - 2 , indo - land fura - 2 have the advantage of wavelength - shift upon binding ca2 + but share a common drawback in that the excitation bands for these indicators occur at uv wavelength ( 340 - 360nm ) , where autofluorescence from cells is high , radiation - damage of cellular structures is serious , the transmission of microscope optics is poor , and the availability of aberration - corrected optics for confocal microscopy is limited 迄今為止,尚未見既可長波激發(fā)、又與ca ~ ( 2 + )結(jié)合后熒光峰位移、且對胞內(nèi)ca ~ ( 2 + )測定具特異性、可在亞細(xì)胞水平進(jìn)行ca ~ ( 2 + )測定的整體性能優(yōu)良的鈣熒光探針及其在生命科學(xué)中的應(yīng)用研究報(bào)道。